Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane
compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and
Bradford-type assay reagents for protein quantitation.
Tested for suitability on agarose gels following immunoelectrophoresis.
Brilliant Blue R can be used for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates.